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Oligonucleotides for antisense technology |
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 Targets for antisense strategies Antisense technology is the use of oligonucleotides to inhibit gene expression through sequence specific hybridization. This process has gained increasing acceptance in the study of gene function and may also lead to new therapies for many human diseases. Inhibition of gene expression by antisense oligonucleotides based on the ability of an oligonucleotide to bind a complementary messenger RNA sequence thus preventing translation of the messenger RNA. Several mechanisms have been shown to mediate the inhibitory functions of oligonucleotides in gene expression. The most important seems to be the physical inhibition of the translation on ribosomes and the destruction of mRNA in RNA/DNA-duplexes by RNase H. An ideal antisense oligonucleotide should bind mRNA specifically and with high affinity. Furthermore, it should be able to penetrate cell membranes and have sufficient resistance to extra- and intracellular nucleases. In these terms, the most successfully used antisense oligonucleotides are: References cited above
Phosphorothioate analogs in which a non-bridging oxygen has been replaced by sulfur have widespread use in molecular biology. The increased resistance of phosphorothioates against nuclease digestion has prompted the consideration of these molecules for medical purposes1-3. In addition to their value as antisense probes, phosphorothioates have also been used for the site specific attachment of reporter groups at the DNA/RNA backbone4 and for preventing degradation of oligonucleotides by 3' |
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| product |
scale |
amount [oD] |
cat. no. |
price/base |
| Antisense phosphorothioate coupling of DNA oligonucleotides |
0.01 µmol |
0.5 - 1 |
5-0410-011 |
2.50 EUR |
|
0.05 µmol |
2.0 - 4 |
5-0410-012 |
2.50 EUR |
|
|
0.20 µmol |
10.0 - 20 |
5-0410-013 |
3.50 EUR |
|
|
1.00 µmol |
50.0 - 100 |
5-0410-014 |
5.00 EUR |
|
|
15.00 µmol |
600.0 - 1200 |
5-0410-015 |
60.00 EUR |
|
| Antisense phosphorothioate coupling of RNA oligonucleotides |
1.00 µmol |
50.0 - 100 |
5-0411-014 |
19.00 EUR |
| setup for orders containing less than 5 ASPTO couplings |
5-50410-010 |
7.50 EUR |
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'5'-exonuclease activities of proof reading DNA polymerases such as Vent and Pfu5. Phosphorothioate-substituted RNA has been used to study specific RNA-Protein interactions6.