IBA : NAPS

Since 1975 it is known that double stranded RNA (dsRNA) can inhibit protein synthesis (1,2). Several recent applications described the use of short dsRNA oligo-nucleotides for gene silencing, i.e. the inhibition of protein synthesis (3-5).

Being a specialist for RNA synthesis we are offering the RNA tools you require for these studies.
Short RNA duplexes are offered ready-to-use, i.e. purified, deprotected and annealed. A negative control sense and antisense RNA can be included on request. There are no additional charges for the number of base pairs. For longer RNA duplexes the costs are based on a price per base pair plus a fixed fee for annealing and purification.

Also available labeled with fluorescent dyes or biotin (order information below)!

Literature

1) Hunter T, Hunt T, Jackson RJ, Robertson HD, 1975 : The characteristics of inhibition of protein synthesis by double-stranded ribonucleic acid in reticulocyte lysates.

2) Clemens MJ Safer B Merrick WC Anderson WF London IM, 1975: Inhibition of protein synthesis in rabbit reticulocyte lysates by double-stranded RNA and oxidized glutathione: indirect mode of action on polypeptide chain initiation. Proc Natl Acad Sci U S A Apr;72(4):1286-90 J Biol Chem Jan 25;250(2):409-17

3) Caplen NJ Parrish S Imani F Fire A Morgan AR, 2001: Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci U S A. 2001 Aug.14;98(17):9742-7.

4) Hamilton AJ Baulcombe DC, 1999: A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 1999 Oct 29;286(5441):950-2

5) Djikeng A Shi H Tschudi C Ullu E, 2001: RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs. RNA 2001 Nov;7(11):1522-30

Order information
see also "how to order"

Order online

product	scale	amount	cat.no.	price
Ready-to-use: RNA duplexes, purified, deprotected and annealed, unlabeled; negative control (corresponding sense and antisense strands) on request	0.2 µmol	20-40 nmol (140-280 µg) ds RNA; 5-10 nmol ssRNA as control	5-0515-113	350.00 EUR
	1.0 µmol	50-100 nmol ds RNA; 10-20 nmol ssRNA as control	5-0515-114	790.00 EUR
Ready-to-use: RNA duplexes with fluorescent label or biotin, purified, deprotected and annealed; negative control (corresponding sense and antisense strands) on request	1.0 µmol	50-100 nmol ds RNA; 10-20 nmol ssRNA as control	5-0516-124	890.00 EUR
Ready-to-use: positive control siRNA (for β-Actin or lamin A/C); unlabeled		5, 10 or 25 nmol	5-0515-142/3 or 4 (lamin A/C)	100.00 EUR 150.00 EUR or 200.00 EUR
		5, 10 or 25 nmol	5-0515-152/3 or 4 (β-Actin)	100.00 EUR 150.00 EUR or 200.00 EUR
Ready-to-use: double stranded negative control RNA (mock), pre-defined, unlabeled		5, 10 or 25 nmol	5-0515-132/3 or 4	100.00 EUR 150.00 EUR or 200.00 EUR
Ready-to-use: double stranded negative control RNA (mock), pre-defined, labeled		25 or 80 nmol	5-0515-163 or 5-0515-164	300.00 EUR or 890.00 EUR

For more antisense products please refer to "Oligonucleotides for Antisense Technology"

Disclaimer: Purchasers of RNA products intended for the use in RNA interference experiments, whether in vitro or in vivo may require a license to one or more of the granted patents and pending patent applications covering the usage of siRNA for gene knockout, target validation and therapeutic development. Particularly relevant are patents granted to Ribopharma AG (EP 1144623 A1) and the Carnegie Institute of Washington (US 6, 506,559 B1) and applications (including WO 01/75164 A2) filed by the Whitehead Institute of Biomedical Research, the Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., the Massachusetts Institute of Technology and the University of Massachusetts Medical Center. If in doubt please contact the owners of the patents directly.

Double stranded RNA for interference studies