pASG-IBA vectors
- High-level expression in E. coli, also for toxic proteins
- Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
- Option for periplasmic expression due to ompA signal sequence
- No catabolite repression - no influence of medium components
- Not influenced by the genetic background – wide choice of E. coli expression strains
- Inexpensive induction with AHT.
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pPSG-IBA vectors
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
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pESG-IBA vectors
- High-level constitutive expression in mammalian cells by the CMV promoter
- Neomycin resistance for generation of stable cell lines
- ColEl ori and ampicillin resistance gene support propagation in E. coli
- BM40 option for secretion of protein into the medium
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pCSG-IBA vectors
- High-level constitutive expression in mammalian cells by the CMV promoter
- extrachromosomal replication due to Epstein Barr Virus replication origin (oriP) and EBNA-1
- prolonged expression of the inserted GOI under G418 selection without the need for making stable cell lines
- ColEl ori and ampicillin resistance gene support propagation in E. coli
- BM40 option for secretion of protein into the medium
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pYSG-IBA vectors
- High-level expression in yeast
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers leu2-d and URA3
- High copy number
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pLSG-IBA vectors
- Cloning and expression of recombinant proteins in insect cells
- Polyhedrin promoter drives high-level expression of the insert
- pUC ori and ampicillin resistance gene support propagation in E. coli
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Destination Vector expression cassettes see here.
Tailor-made backbones on request.
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| Please download the list of StarGate Acceptor Vectors here |
