Innovative software for StarGate® Entry Cloning Primer
Make use of the easy-to-use software, the StarPrimer D´Signer 3.0.0.3, to facilitate the design of primers for the ENTRY cloning step to create the Donor Vector.
The software is also very well suited for the design of primers in combination with a site-directed mutation approach. In this case it is used to design primers for introducing mutations into your gene of interest which then is transferred into a StarGate ENTRY vector to create a Donor Vector carrying the mutated gene of interest.
This software does not only help with the primer design, it will also guide you step by step through the whole PCR process.
For using the new StarPrimer D´Signer 3.0.0.3 software please click here
Requirements to run StarPrimer D´Signer Software.
- Installation of Microsoft Silverlight is necessary to run the StarPrimer D´Signer software.
- System requirements:
- For PC (Win 2000 SP4, Win XP SP 2/3, Windows Vista and Windows 7)
- For Apple Macintosh ( Intel Processor and MAC OS X 10.4.11 )
Browser should be current version of Internet Explorer, Firefox or…
(If problems with the software occur please contact support@iba-go.com).
**Please note IBA has changed the Entry Cloning Step by May 2010. If you have used StarGate in the past please check the sequence of your Entry Cloning Primers with the new StarPrimer D´Signer 3.0.0.3**
PTO Protected Cloning Primers
For StarGate® cloning we recommend the use of 3’ phosphorothioate (PTO) protected oligonucleotides which are protected against the exonuclease activity of Proof Reading Polymerases. These Primers can be directly ordered at IBA’s Gene TAGnology division, which offers excellent custom service for synthesis of a wide range of high quality nucleic acid specialties at competitive prices.
For further information about PTO Protected Cloning Primers please visit our Gene TAGnology department.
Place oligo orders here. |
