Evaluate the best protein-tags and hosts for the expression of your gene of interest very fast and easy
StarGate Cloning Features:
Donor Vector as universal basic element
Introduction
Efficient procedures to generate functional recombinant proteins or protein complexes are of key importance in state-of-the-art life sciences. Many tools like various expression hosts (bacteria, yeast, insect and mammalian cells), promoters and affinity tags are currently available to express, purify, detect or immobilize recombinant proteins. Due to the diverse nature of proteins, however, it is impossible to predict which combination of these tools will perform best. Therefore, many have to be tried to identify the optimal solution (see picture below).
To systemize and accelerate this initial search which is crucial for successful subsequent proteomic research, we have developed the StarGate Cloning System. StarGate offers rapid and highly efficient subcloning of an arbitrary gene - initially cloned into a Donor Vector - to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate Destination Vectors. The latter enable the efficient expression of your protein with various features (e.g. different tags and different promoters) in different hosts (see picture above). Due to its standardized protocol StarGate is also very well suited for high-throughput applications.
Expression profile for a certain gene expressed from different Acceptor Vectors
This Figure illustrates the requirement for an expression system like StarGate enabeling the fast evaluation of different expression conditions leading to optimal expression results.
Expression of the 14kDa protein azurin with different tags and promoters
(For details on the Figure above please click here)
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