Protein Complex Purification and
 Protein:Protein Interaction Analysis



- based on Strep-tag® and One-STrEP-tag technology



"We chose the One-STrEP-tag for the following reasons:
The One-STrEP system provides a reliable, efficient and rapid one-step purification method useful for isolation of protein complexes from stable human cell lines. It is compatible with high stringency washing to obtain complexes of high purity and it is reasonably low cost compared to other systems."

Anja Groth, Biotech Research and Innovation Centre,
University of Copenhagen

Application examples

Properties / Benefits

  • Strep-tag®II and One-STrEP-tag have a neutral amino acid composition
  • Purification of recombinant proteins to over 99% purity in a single step
  • Very low tendency of Strep-Tactin® to bind other proteins non-specifically
  • Highly specific Strep-tag®II (OneSTrEP-tag):Strep-Tactin® interaction
  • Specific competitive elution with minute amounts of (desthio-)biotin in the physiological wash buffer


Strep-tag II: SAWSHPQFEK

One-STrEP-tag: SAWSHPQFEK(GGGS)2GGSAWSHPQFEK

As a conclusion, extremely efficient and fast Strep-tag®II and One-STrEP-tag are reliable tools to meet the challenge of isolating protein complexes at high purity without loosing transient binders.


References:

  1. Schmidt & Skerra, 2007, Nature Protocols 2: 1528-35
  2. Ostermeier et al., 1997, PNAS 94: 10547-10553
  3. Rigaut et al., 1999, Nature Biotech. 17: 1030-103
  4. Juntilla et al., 2005, Proteomics 5: 1199-1203
  5. Johansen et al., 2008, J Cell Sci 121: 854-864
  6. Groth et al. 2007 Science 318: 1928-1931
  7. Schaffitzel & Ban, 2007, J Struct Biol 158: 463-471
  8. Morita et al., 2007, EMBO J. 26: 4215-4227
  9. Morita et al., 2007, Cell Host & Microbe 2: 19-28
  10. Gloeckner et al. 2007 Proteomics 7, 4228-4234
  11. Herzberg et al. 2007 Proteomics 7, 4032-4035
  12. Jarchow et al., 2008, Proteomics, 8: 4987-4994
  13. Glatter and Wepf , 2009, Molecular Systems Biology 5: 237
  14. Pegoraro, 2009, Nature Cell Biology 11 : 1261-1267
  15. Amako et al., 2009 J Virol.; 83(18): 9237–9246
  16. Gianni et al., 2009 J. Biol. Chem. 284: 17370-17382,
  17. Rameix-Welti et al., 2009 J.Virol. 83(3):1320–1331
  18. Neumann et al., 2009 Immunological Reviews 232:135-149
  19. Oellerich et al., 2009 Molecular Cell Proteomics 8(7):1738-50
  20. Bekker-Jensen et al.,  2010 Nature cell biology 12(1)
  21. Jasencakova et al., 2010 Molecular Cell 37: 736–743

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